Can organoids be cryopreserved or stored at low temperatures?
Yes. Organoids can be temporarily stored at low temperatures (e.g., 4 ℃) for short-term use, while long-term storage relies on cryopreservation with type-specific optimized cryopreservation media. Most patient-derived organoids can be successfully cryopreserved. However, highly terminally mature iPSC‑derived organoids such as cerebral organoids show poor post-thaw viability after cryopreservation. Pretreatment with a ROCK inhibitor (e.g., Y-27632) before freezing is recommended to improve post-thaw cell viability. All patient-derived organoids supplied by Cytrix are cryopreserved using validated protocols, achieving post-thaw viability of over 80% for rapid culture recovery.