Organoids can be derived from patient-derived normal adult or tumor tissues (patient-derived organoids, PDOs) or pluripotent stem cells, including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). Isolation and expansion of resident stem cells are critical for self-renewal of tissue-derived organoids, while stem cell differentiation drives pluripotent-stem-cell-derived organoid formation. Additional key factors include qualified batches of GFR-reduced Matrigel matrix, standardized passaging techniques, and high-quality culture media and reagents. Cytrix provides fully validated Organoid Culture Media and tissue-specific dissociation reagents to ensure reproducible organoid generation.