Yes. Within a suitable range, studies (e.g., Calandrini et al., 2022) have shown that the growth rate and size of organoids are positively correlated with the number of cells seeded. Seeding at an appropriate density enhances organoid formation efficiency. However, excessively high seeding density may lead to increased cell death in the core region of the Matrigel dome._x000D_
Calandrini C, Drost J, et al. Normal and tumor-derived organoids as a drug-screening platform for tumor-specific drug vulnerabilities. STAR Protoc. 2022;3(1):101079.