R-004
Recombinant Human EGF Protein, Fc Tag
Recombinant Human EGF Protein, Mouse IgG2a Fc Tag is a recombinant growth factor fused with a mouse IgG2a Fc domain, improving stability and handling while maintaining potent EGFR signaling activity. EGF is a core component of organoid media for epithelial tissues, including intestinal, colonic, gastric, and pancreatic organoids, where it supports stem cell proliferation and differentiation. The Fc fusion format enhances half-life and facilitates purification, ensuring reproducible results in signaling assays and organoid-based disease modeling.
Specifications
- cat no
- R-004
- shipping info
- Ice
- application
- For research use only.
- product type
- Growth factor
- product description
- Recombinant Human EGF Protein, Mouse IgG2a Fc Tag is a recombinant growth factor fused with a mouse IgG2a Fc domain, improving stability and handling while maintaining potent EGFR signaling activity. EGF is a core component of organoid media for epithelial tissues, including intestinal, colonic, gastric, and pancreatic organoids, where it supports stem cell proliferation and differentiation. The Fc fusion format enhances half-life and facilitates purification, ensuring reproducible results in signaling assays and organoid-based disease modeling.
- endotoxin level
- Less than 0.1 EU per μg by the LAL method / rFC method.
- sterility
- Negative
- stability and storage
- Store frozen at -20 °C (short-term) or -80°C (long-term)
- molecular weight
- 33.1 kDa
- mycoplasma
- Negative
- purity
- ≥ 95% as determined by SDS-PAGE
- expression system
- Human 293 cells (HEK293)
- formulation
- Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
- reconstitution
- Please refer to the Certificate of Analysis (CoA) for specific reconstitution instructions.