Recombinant Human EGF Protein, His Tag
Recombinant Human EGF Protein, His Tag to facilitate affinity purification and analytical detection. It is produced in E. coli and exhibits strong bioactivity comparable to native EGF. The His tag allows easy incorporation into ELISA, western blotting, and protein interaction assays, while preserving its ability to activate EGFR-mediated signaling cascades. The product is endotoxin-controlled and purity-tested to ensure consistency across experimental batches. It is widely applicable in 2D and 3D epithelial culture systems, organoid development workflows, and studies requiring traceable EGF variants. Each lot is validated to support robust cell proliferation and functional tissue development in vitro.Used to stimulate epithelial cell growth in culture, support organoid self-organization, and model EGFR activation in disease systems.
Specifications
- cat no
- R-002
- shipping info
- Ice
- application
- For research use only.
- product type
- Growth factor
- product description
- Recombinant Human EGF Protein, His Tag to facilitate affinity purification and analytical detection. It is produced in E. coli and exhibits strong bioactivity comparable to native EGF. The His tag allows easy incorporation into ELISA, western blotting, and protein interaction assays, while preserving its ability to activate EGFR-mediated signaling cascades. The product is endotoxin-controlled and purity-tested to ensure consistency across experimental batches. It is widely applicable in 2D and 3D epithelial culture systems, organoid development workflows, and studies requiring traceable EGF variants. Each lot is validated to support robust cell proliferation and functional tissue development in vitro.Used to stimulate epithelial cell growth in culture, support organoid self-organization, and model EGFR activation in disease systems.
- endotoxin level
- Less than 0.1 EU per μg by the LAL method / rFC method.
- sterility
- Negative
- stability and storage
- Store frozen at -20 °C (short-term) or -80°C (long-term)
- molecular weight
- 8.3 kDa
- mycoplasma
- Negative
- purity
- ≥ 95% as determined by SDS-PAGE
- expression system
- Human 293 cells (HEK293)
- formulation
- Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
- reconstitution
- Please refer to the Certificate of Analysis (CoA) for specific reconstitution instructions.