R-009
Recombinant Human R-Spondin 1 Protein, His Tag
Recombinant Human R-Spondin 1 Protein, His Tag is a recombinant full-length Wnt signaling enhancer fused with a His tag. It strongly potentiates Wnt/β-catenin activity, essential for epithelial stem cell proliferation and organoid culture. R-Spondin 1 is required in most epithelial organoid protocols, particularly for intestinal and colonic organoids, where it sustains long-term expansion. The His tag supports assay flexibility and reliable purification, ensuring reproducibility across organoid and developmental biology applications.
Specifications
- cat no
- R-009
- shipping info
- Ice
- application
- For research use only.
- product type
- Growth factor
- product description
- Recombinant Human R-Spondin 1 Protein, His Tag is a recombinant full-length Wnt signaling enhancer fused with a His tag. It strongly potentiates Wnt/β-catenin activity, essential for epithelial stem cell proliferation and organoid culture. R-Spondin 1 is required in most epithelial organoid protocols, particularly for intestinal and colonic organoids, where it sustains long-term expansion. The His tag supports assay flexibility and reliable purification, ensuring reproducibility across organoid and developmental biology applications.
- endotoxin level
- Less than 1.0 EU per μg by the LAL method / rFC method.
- sterility
- Negative
- stability and storage
- Store frozen at -20 °C (short-term) or -80°C (long-term)
- molecular weight
- 28.7 kDa
- mycoplasma
- Negative
- purity
- ≥ 95% as determined by SDS-PAGE
- expression system
- Human 293 cells (HEK293)
- formulation
- Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
- reconstitution
- Please refer to the Certificate of Analysis (CoA) for specific reconstitution instructions.