Recombinant Human Shh Protein, His Tag, Premium Grade
Recombinant Human Shh Protein, His Tag, Premium Grade is a high-purity, His-tagged recombinant form of the Sonic Hedgehog (SHH) protein, a key regulator of cell differentiation, tissue patterning, and developmental processes. SHH is expressed in the fetal intestine, liver, lung, and kidney, but is not typically present in adult tissues. The SHH protein undergoes autocleavage into its active N-product and inactive C-product, with the N-product being responsible for signaling activity. The N-product binds to the Patched (PTC) receptor and interacts with Smoothened (SMO) to initiate the transcription of target genes. The His tag enables efficient purification and detection, making this protein an ideal choice for studies involving cell signaling, organoid development, and the study of SHH receptor interactions.
Specifications
- cat no
- R-048
- shipping info
- Ice
- application
- For research use only.
- product type
- Growth factor
- product description
- Recombinant Human Shh Protein, His Tag, Premium Grade is a high-purity, His-tagged recombinant form of the Sonic Hedgehog (SHH) protein, a key regulator of cell differentiation, tissue patterning, and developmental processes. SHH is expressed in the fetal intestine, liver, lung, and kidney, but is not typically present in adult tissues. The SHH protein undergoes autocleavage into its active N-product and inactive C-product, with the N-product being responsible for signaling activity. The N-product binds to the Patched (PTC) receptor and interacts with Smoothened (SMO) to initiate the transcription of target genes. The His tag enables efficient purification and detection, making this protein an ideal choice for studies involving cell signaling, organoid development, and the study of SHH receptor interactions.
- endotoxin level
- Less than 0.2 EU per μg by the LAL method / rFC method.
- sterility
- Negative
- stability and storage
- Store frozen at -20 °C (short-term) or -80°C (long-term)
- molecular weight
- 20.4 kDa
- mycoplasma
- Negative
- purity
- ≥ 90% as determined by SDS-PAGE.
- expression system
- Human 293 cells (HEK293)
- formulation
- Lyophilized from 0.22 μm filtered solution in PBS, 60 mM NaCl, pH7.4 with trehalose as protectant.
- reconstitution
- Please refer to the Certificate of Analysis (CoA) for specific reconstitution instructions.